Biomarkers are measurable indicators of health status or disease state that can be used for diagnosis and may help guide patient treatment strategies. Enzyme-linked immunosorbent assays (ELISA) and other many clinical techniques currently used for measuring biomarker proteins lack sensitivity, demand high analysis cost, are often not well-suited for measuring multiple biomarkers in a single sample, and require long analysis times. Here, we demonstrate simple, low-cost 3D-printed flow-through devices with integrated electrodes modified with gold nanoparticles (AuNPs) for electrochemical immunoassays of S100B, a biomarker protein related to conditions like skin cancer and brain injuries. Flow-through devices are fabricated from photocurable-resin using a desktop digital light processing (DLP) projector-based 3D printer to produce 500-800 µm square cross-sectional fluidic channels. Threaded ports at the ends and center of the channel are included in the device design for connecting commercially available fittings for fluid delivery and integrating low-cost graphite electrodes for electrochemical biosensing.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-4930 |
| Date | 01 August 2018 |
| Creators | Alabdulwaheed, Abdulhameed |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations |
| Rights | Copyright by the authors. |
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