The effect of ASA [(Aldrich, Steinheim, Germany); 80 g L-1 stock solution in
absolute ethanol (Merck, Darmstadt, Germany)] on yeast growth was assessed in test
tubes containing 5 mL of YNB (6.7 g L-1; Difco Laboratories, Detroit, Michigan, USA)
broth supplemented with 2 % glucose (Saarchem, Wadeville, South Africa) (Petrou &
Shanson, 2000). The tubes were incubated aerobically with 2 day old cultures on YM
agar while agitating on a Rollordrum (30 oC for 4 days) according to the assimilation
tests in liquid medium protocol (Yarrow, 1998). All yeasts were subjected to the following
ASA concentration gradient: 0 mM, 1 mM, 2 mM, 3 mM, 4 mM and 5 mM ASA. Ethanol
(ETOH) control (equivalent to the ethanol volume used to reconstitute 5 mM ASA) as
well as a negative control (i.e. no ASA, ethanol or inoculum) were included. Growth was
determined visually using a white card containing black lines as described by Yarrow
(1998). Here, +++ indicates good growth (no black lines visible), ++ indicates growth
(black lines just visible) and + indicates weak or no growth (black lines similar to that of
inoculated tubes at the start of growth).
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-08222008-090131 |
| Date | 22 August 2008 |
| Creators | Sebolai, Olihile Moses |
| Contributors | Dr CH Pohl, Prof PWJ van Wyk, Prof JLF Kock |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-08222008-090131/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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