In this study crude soy oil was extracted from the soy flour by hexane solvent. The crude oil was refined using a refining procedure similar to the one in edible oil industries, which included degumming, neutralizing, and bleaching. As the result, the eight groups of the oils and the gums were obtained. The compositions of fatty acids and tocopherols in the eight groups of samples were analyzed using GC-FID and HPLC, respectively. The antioxidant activities of the samples were analyzed by two chemical models, cholesterol and DHA. The results showed that ã- and ä-tocopherols may not be the main antioxidants of the crude oil when studied by the two models. The analyses for the antioxidant activities indicated that gum-1 had the highest antioxidant activity among the samples. The gum-1 was fractionalized by a silica gel column and three fractions were obtained. The antioxidant activities of the fractions were analyzed by the cholesterol model. The result indicated that the ethyl acetate/hexane fraction had the highest activity among the fractions. The fraction was further analyzed and fractionalized by RE-HPLC using a two-step elution scheme. As a result, a RE-HPLC fraction containing two individual peaks was demonstrated higher antioxidant activity. A HPLC peak was identified as a phytosterol (plant cholesterol) by searching a GC-MS database.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-05012006-115234 |
| Date | 03 May 2006 |
| Creators | Yuan, Xianglong |
| Contributors | Cristina M. Sabliov, Witoon Prinyawiwatkul, Zhimin Xu |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-05012006-115234/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached herein a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below and in appropriate University policies, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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