The genus Dipodascopsis was extensively studied with regards to reproductive
cycles as well as the presence, distribution and function of 3-OH oxylipins. Most of
this research was carried out on D. uninucleata var. uninucleata (Canadian strain) as
well as D. tóthii. However, little is known concerning D. uninucleata var. uninucleata
isolated from South African soil, as well as D. uninucleata var. wickerhamii.
Consequently, using gas chromatography-mass spectrometry, electron microscopy
and confocal laser scanning microscopy, the two varieties were compared regarding
their morphologies, oxylipin production, mitochondrial activity as well as life cycles
and ascospore release. According to literature, the two varieties differ only in their
ability to assimilate certain carbon sources. During this study, differences in
ascospore size as well as differences in ascospore clustering, after release, was
observed. Furthermore, differences in the type of 3-OH oxylipin produced by the two
varieties, also existed. 3-OH oxylipin production was found to be associated mainly
with the sexual stage and concentrated in the ascus surrounding the ascospores, in
both varieties. Furthermore, increased mitochondrial activity was also observed
during the sexual stage and found to be concentrated in close vicinity of the
ascospores. Since mitochondria produce 3-OH oxylipins, it is suggested that the
increased activity during sexual development would be to aid in the production and
release of the ascospores, as well as the accumulation of these 3-OH oxylipins. In
addition, acetylsalicylic acid was found to inhibit the production of 3-OH oxylipins by
probably decreasing mitochondrial activity resulting in the inhibition of ascospore
release.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-05152009-091056 |
| Date | 18 May 2009 |
| Creators | Goldblatt, Monique E |
| Contributors | Dr CH Pohl, Prof PWJ van Wyk, Prof JLF Kock |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-05152009-091056/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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