In this study, the construction of a forecasting model, using intracellular fatty acid
composition as indicator, was attempted to assist in the search for yeasts capable of
producing 3-hydroxy oxylipins. In order to achieve this, it was first attempted to establish
a database mapping the distribution of fatty acids (FAs) associated with the neutral-,
glyco- and phospholipid fractions of the 10 species representing the genus
Saccharomycopsis. It was possible to identify nine of the 10 species i.e.
Saccharomycopsis capsularis, S. crataegensis, S. fibuligera, S. javanensis, S. malanga,
S. schoenii, S. selenospora, S. synnaedendra and S vini with the exception of S.
fermentans. Saccharomycopsis crataegensis was unique since it produced by far the
highest percentage neutral lipids (52.4% w/w) while S. schoenii produced the highest
percentage phospholipids (35.9% w/w). All strains produced palmitic- (16:0), stearic-
(18:0), oleic- (18:1) and linoleic acid (18:2) in all lipid fractions analysed. The major FAs
produced were 18:1 and 18:2, while palmitoleic- (16:1) and a-linolenic acid [18:3 (w-3)]
varied between species. Saccharomycopsis capsularis produced the highest percentage
18:2 in the neutral lipid fraction while S. crataegensis, S. malanga and S. selenospora
produced the highest percentages of 18:1, 18:0 and, 18:3 (w-3) respectively, in the
neutral lipids. Saccharomycopsis vini produced the lowest percentage 16:0 in this
fraction. Saccharomycopsis fibuligera and S. schoenii produced the highest percentages
of 16:0 and 18:2 respectively in the glycolipid fraction. Saccharomycopsis javanensis
and S. synnaedendra produced the highest percentages of 18:1 and 16:1 respectively in
the phospholipid fraction. Although it was possible to differentiate between most species using this phenotypic character, these FAs could not be used to predict what kind of 3-OH
oxylipins these species are capable of producing.
Saccharomycopsis fermentans (novel unidentified 3-OH oxylipin), S. malanga (3-OH
16:0), S. synnaedendra (3-OH 16:0, 3-OH 17:0, 3-OH 18:0, 3-OH 18:1, 3-OH 19:0,
3-OH 19:1, 3-OH 20:0, 3-OH 22:0) and S. vini (3-OH 9:1, 3-OH 10:1) could be
separated using this character. Although, S. capsularis and S. javanensis both produced
3-OH 9:1, fatty acids with uneven carbon atoms which may serve as precursors could
not be detected in the neutral-, glyco- or phospho-lipid fractions.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-07052005-133803 |
| Date | 05 July 2005 |
| Creators | Sebolai, Olihile Moses |
| Contributors | Prof JLF Kock, Prof MS Smit, Prof PWJ van Wyk |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-07052005-133803/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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