Serum-free mouse embryo (SFME) cells are derived
in medium in which serum is replaced with growth
factors and other supplements. They display unusual
properties. They do not lose proliferative potential
or show gross chromosomal aberration upon extended
culture, they depend on epidermal growth factor (EGF)
for survival, and are reversibly growth inhibited by
plasma and serum. In the presence of transforming
growth factor beta (TGF-β) SFME cells express the
astrocyte marker, glial fibrillary acidic protein
(GFAP).
The growth inhibitory activity of human plasma
on serum-free mouse embryo cells was investigated.
Human plasma did not inhibit SFME cells transformed
with the human Ha-ras oncogene. The activity was
present in delipidated plasma and was not dialyzable
against 1 M acetic acid. The activity could be
precipitated by methanol, bound to concanavalin Aagarose
and was retarded by Sephadex G-50 in 200 mM
acetic acid. A fifty to hundred fold purification was
achieved, although the differential inhibition of
untransformed versus transformed cells was lost in the
course of the purification.
Using the technique of differential
screening of a cDNA library a calf serum- and TGF -β-regulated
mRNA species was identified in SFME cells.
This mRNA was approximately 8.5 kilobases in size and
brain-specific. Picomolar quantities of TGF-β caused
an increase of this message in SFME cells within four
hours. This increase was reversed when TGF-β was
removed from the culture medium. / Graduation date: 1993
| Identifer | oai:union.ndltd.org:ORGSU/oai:ir.library.oregonstate.edu:1957/36112 |
| Date | 05 June 1992 |
| Creators | Varga Weisz, Patrick D. |
| Contributors | Barnes, David W. |
| Source Sets | Oregon State University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
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