The objective of this study was to uncover molecular and cellular dynamics in spermatozoa from Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics, membrane integrity, and DNA fragmentation were assessed. Data was analyzed using principal component analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P = 0.029), nonragmented DNA (P = 0.018), post-thaw viability (P < 0.001) in comparison to those of the Poor bulls. The Good bulls also had a faster average path velocity (VAP; P = 0.017), straight-line velocity (VSL; P = 0.036), a greater distance average path (DAP; P = 0.006) and distance straight line (DSL; P = 0.011). No differences were found in total antioxidant capacity (TAC), number of live cells or other kinetic parameters between spermatozoa from Good and Poor groups, showing that no one characteristic can be used to determine cryotolerance.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-5566 |
| Date | 07 August 2020 |
| Creators | Gilmore, Alicia |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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