Little is known about the molecular genetic variation of Ground Pangolin populations in
South Africa. In this study it was attempted to assess the genetic diversity of the
populations, but this could not be achieved due to insufficient crossâspecies markers
amplification. It should, however, be emphasized that the molecular work done in this
study is novel, and that the results found during this research are key foundations for
future studies. During sample collection it was found that three main populations of
Ground Pangolins exist in South Africa in the Eastern, Western and Central parts of the
country. Isolation protocols have been optimized, and it has been shown that noninvasive
samples yield good quality and quantity DNA that is usable for downâstream
applications and perform as well as invasive samples. The PCR protocol was optimized,
and the results from the optimization chapter will be of assistance when speciesâspecific
markers are optimized. This study has also shown the need for the development of
speciesâspecific markers, and the use of said markers will give a better indication of the
genetic diversity of the Ground Pangolin populations in South Africa. From the
statistical analysis it would seem that there are some correlations between the three
sampling localities which may indicate a population divergence at some point. Based on
the genetic diversity results, it appears that the diversity within Ground Pangolin
populations is much lower based on the markers tested than in Malayan Pangolin
populations. This will however have to be confirmed with speciesâspecific markers.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ufs/oai:etd.uovs.ac.za:etd-08202014-092131 |
| Date | 20 August 2014 |
| Creators | de Beer, Christle |
| Contributors | Prof R Jansen, Dr K Ehlers, Dr D Dalton, Prof A Kotze |
| Publisher | University of the Free State |
| Source Sets | South African National ETD Portal |
| Language | en-uk |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.uovs.ac.za//theses/available/etd-08202014-092131/restricted/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University Free State or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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