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Previous issue date: 2017-03-27 / Peripheral nerve injury continues to be an important research topic in the scientific community as it may cause lifelong disability. Biocompatible polymers are materials potentially capable of aiding the regeneration of peripheral nerves being used for the production of biocompatible tubes. The aim of this work is to prepare and characterize polymeric nanocomposites based on polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA) and polypyrrole nanofibers (PPy) capable of acting as a guidewire in the regeneration of peripheral nerves. PPy was synthesized by oxidative chemical polymerization with p-toluenesulphonic acid monohydrate (PTSA) as a doping agent. PCL:PLGA blends films and PCL:PLGA:PPy nanofibers nanocomposites films were prepared by the solvent casting method, in the ratio of PCL:PLGA 100:0, 90:10, 80:20 and 70:30 (m/m); to the films with nanoload were added 10% PPy. In order to characterize the films, the following techniques were used: SEM, DSC, TGA, determination of electric conductivity and contact angle, citotoxicity test and hydrolytic degradation test, in vitro, based on ASTM F1635-11 standards. The PPy nanofibers presented electrical conductivity equal to 2.0.10-1 S.cm-1. The presence of PLGA and PPy did not change, meaningfully, the thermal properties of the films. However, in the degradation process, there was a tendency to a greater loss of mass for the blends with higher percentage of PLGA when submitted to longer incubation periods (150 days); when PPy was added to these blends, mass loss occurred in shorter periods (90 days). The nanocomposites films showed nontoxic and porous morphology surface, with hydrophilic intermediary character, good thermal stability and adequate degradation time for potential use in the treatment of injury in peripheral nerves. / Les?o de nervos perif?ricos continua sendo um importante tema de pesquisas no meio cient?fico, podendo causar defici?ncia no paciente por toda a vida. Pol?meros biocompat?veis s?o materiais potencialmente capazes de auxiliarem a regenera??o de nervos perif?ricos sendo utilizados para a produ??o de tubos biocompat?veis. O objetivo deste trabalho ? preparar e caracterizar nanocomp?sitos polim?ricos baseados em policaprolactona (PCL), poli(?cido l?ctico-co-glic?lico) (PLGA) e nanofibras de polipirrol (PPy) capazes de atuarem como conduto guia na regenera??o de nervos perif?ricos. PPy foi sintetizado via polimeriza??o qu?mica oxidativa com ?cido p-toluenosulf?nico monohidratado (APTS) como agente dopante. Foram preparados filmes de blendas PCL:PLGA e de nanocomp?sitos PCL:PLGA:nanofibras de PPy pelo m?todo de evapora??o de solvente, nas raz?es de PCL:PLGA 100:0, 90:10, 80:20 e 70:30 (m/m); aos filmes com a nanocarga, foram adicionados 10% de PPy. Para caracteriz?-los, foram utilizadas as t?cnicas: MEV, DSC, TGA, determina??o da condutividade el?trica e do ?ngulo de contato, teste de citotoxicidade e de degrada??o hidrol?tica, in vitro, com base na norma ASTM F1635-11. As nanofibras de PPy apresentaram condutividade el?trica igual a 2,0.10-1 S.cm-1. A presen?a de PLGA e de PPy n?o modificou, significativamente, as propriedades t?rmicas dos filmes. Por?m, no processo de degrada??o, houve uma tend?ncia ? maior perda de massa para as blendas com maior percentual de PLGA quando submetidas a maiores tempos de incuba??o (150 dias); ao adicionar PPy ?s blendas, a perda de massa ocorreu em menores tempos (90 dias). Os filmes dos nanocomp?sitos apresentaram superf?cie at?xica e de morfologia porosa, com car?ter hidrof?lico intermedi?rio, boa estabilidade t?rmica e tempo de degrada??o adequado para o potencial uso no tratamento de les?es em nervos perif?ricos.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7591 |
| Date | 27 March 2017 |
| Creators | Ferreira, Cristina Lorenski |
| Contributors | Basso, Nara Regina de Souza |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais, PUCRS, Brasil, Faculdade de Engenharia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7432719344215120122, 600, 600, 600, -655770572761439785, 4518971056484826825 |
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