by Choy Wing Yiu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 115-121). / DESCRIPTIVE NOTE --- p.iii / ACKNOWLEDGEMENTS --- p.v / Chapter CHAPTER ONE: --- BRIEF REVIEW OF THE WATR-CPMG AND OTHER SOLVENT SUPPRESSION METHODS --- p.1 / Chapter 1.0 --- Introduction --- p.1 / Chapter 1.1 --- Some Common Solvent Suppression Techniques --- p.2 / Chapter 1.1.1 --- Brief Summary of Some Common Solvent Suppression Techniques --- p.2 / Chapter 1.1.2 --- Criteria for the Evaluation of Solvent Suppression Methods --- p.8 / Chapter 1.2 --- Application of the WATR-CPMG Method in Solvent Suppression --- p.10 / Chapter 1.2.1 --- The CPMG Pulse Sequence --- p.10 / Chapter 1.2.2 --- The Theory of Solvent Suppression by WATR Method --- p.12 / Chapter 1.3 --- Scope of this Thesis --- p.15 / Chapter CHAPTER TWO: --- OPTIMIZATION OF WATR-CPMG PARAMETERS FOR QUANTITATIVE ANALYSIS IN DILUTE AQUEOUS SOLUTION --- p.19 / Chapter 2.1 --- Investigation of the WATR-CPMG Parameters --- p.19 / Chapter 2.1.1 --- Use of Ammonium Chloride as Proton Exchange Reagent --- p.19 / Chapter 2.1.2 --- The pH Dependence of the Proton Exchange Process --- p.21 / Chapter 2.1.3 --- Relaxation Delay Time --- p.27 / Chapter 2.1.4 --- Measurement of T1 of Protons In Aqueous Solution --- p.31 / Chapter 2.1.5 --- The position of the Carrier Frequency --- p.41 / Chapter 2.1.6 --- Modelling of the Off-Resonance Effect --- p.46 / Chapter 2.1.7 --- The Tau Value Used in the WATR-CPMG Method --- p.51 / Chapter 2.2 --- The Setting of Acquisition Parameters and Data Processing --- p.57 / Chapter 2.2.1 --- Digital Resolution and Spectral Width --- p.57 / Chapter 2.2.2 --- The Setting of Receiver Gain --- p.60 / Chapter 2.3 --- Summary --- p.61 / Chapter CHAPTER THREE: --- APPLICATION OF THE WATR-CPMG METHOD TO THE QUANTITATIVE ANALYSIS OF GLUCOSE IN BLOOD PLASMA AND DILUTE AQUEOUS SOLUTION --- p.62 / Chapter 3.0 --- Introduction --- p.62 / Chapter 3.1 --- Experimental Section --- p.62 / Chapter 3.1.1 --- Characterization of the Metabolites in Blood Plasma --- p.62 / Chapter 3.1.2 --- Reagents --- p.66 / Chapter 3.1.3 --- Solution Preparations --- p.66 / Chapter 3.1.4 --- Measurement of Plasma Glucose by Glucose Oxidase Method --- p.67 / Chapter 3.1.5 --- NMR Measurement --- p.68 / Chapter 3.1.6 --- HPLC Measurement --- p.78 / Chapter 3.2 --- Results and Discussion --- p.79 / Chapter 3.2.1 --- Analysis of Results --- p.79 / Chapter 3.2.2 --- Discussion --- p.84 / Chapter 3.2.3 --- Conclusion --- p.85 / Chapter CHAPTER FOUR: --- HOMODECOUPLED WATR-CPMG METHOD --- p.86 / Chapter 4.0 --- Introduction --- p.86 / Chapter 4.1 --- Application of Combined Time Sharing Homodecoupling WATR-CPMG Method for Quantitative Analysis in Aqueous Solution --- p.87 / Chapter 4.1.1 --- The Time Sharing Homonuclear Decoupling and Average Hamiltonian Theory --- p.87 / Chapter 4.1.2 --- Homodecoupling in Aqueous Solution --- p.89 / Chapter 4.2 --- Experimental --- p.90 / Chapter 4.2.1 --- The Preliminary Investigation --- p.90 / Chapter 4.2.2 --- Homodecoupl ing in 30%D20/70%D20 Solution --- p.97 / Chapter 4.2.3 --- Homodecoupling-CPMG (HDCPMG) in Quantitative Analysis --- p.102 / Chapter 4.3 --- Conclusion --- p.110 / Chapter CHAPTER FIVE: --- CONCLUSION --- p.113 / BIBLIOGRAPHY --- p.115 / APPENDIX I The Computer Simulation of the Off-Resonance Effect on the Water Signal Suppression --- p.122 / APPENDIX II The Computer Simulation Program --- p.125 / APPENDIX III The Simulation Data --- p.129
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319205 |
| Date | January 1993 |
| Contributors | Choy, Wing Yiu., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xvii, 132 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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