Differentiation and gene expression in the oomycete Achlya ambisexualis ( strain E87) were studied during three responses, sexual antheridium formation, asexual sporangium formation and heat shock. Polyacrylamide gel electrophoresis of proteins labelled in vivo and in cell free systems directed by poly(A) mRNA showed that during formation of antheridia only one change was detectable in the proteins synthesized. No detectable changes were observable in the translatable poly(A) RNA population indicating that post-translational events may be required for differentiation. During early sporangium formation several proteins showed changes in relative rates of synthesis. These correlate with similar changes in cell free translation products suggesting a transcriptional level of regulation. Nucleic acid hybridization analyses of mRNA populations indicated that many new sequences appear late and accumulate in the spore. These late transcripts are probably utilized during spore germination. During heat shock several proteins showed rapid increases in rates of synthesis; these seem to be controlled at the level of transcription.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.77135 |
| Date | January 1981 |
| Creators | Gwynne, David I. (David Ivor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biology) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000139593, proquestno: AAINK58032, Theses scanned by UMI/ProQuest. |
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