Chum Wing Yan, Winnie. / Thesis submitted in 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 157-178). / Abstracts in English and Chinese. / English Abstract --- p.i / Chinese Abstract --- p.iii / Acknowledgements --- p.v / Abbreviations --- p.vi / Table of Contents --- p.vii / List of Figures --- p.x / List of Tables --- p.xiii / Chapter Chapter One --- Literature Review / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Life cycle --- p.2 / Chapter 1.3 --- Nutritional value --- p.4 / Chapter 1.4 --- Medicinal value --- p.4 / Chapter 1.4.1 --- Antitumor ability --- p.5 / Chapter 1.4.2 --- Antimicrobial ability --- p.5 / Chapter 1.4.3 --- Hypocholesterolaemic effect --- p.6 / Chapter 1.4.4 --- Anti-viral effect --- p.6 / Chapter 1.4.5 --- Anticaries effects --- p.7 / Chapter 1.6 --- Commercial value --- p.7 / Chapter 1.6.1 --- Biodecolorization --- p.7 / Chapter 1.6.2 --- Bioconversion --- p.7 / Chapter 1.6.3 --- Biodegradation --- p.8 / Chapter 1.6.4 --- Indicator --- p.9 / Chapter 1.7 --- Cultivation --- p.9 / Chapter 1.8 --- Content --- p.10 / Chapter 1.9 --- Transformation --- p.11 / Chapter 1.10 --- Gene regulation for growth and fruiting body development --- p.13 / Chapter 1.11 --- Serial Analysis of Gene Expression --- p.18 / Chapter 1.11.1 --- Introduction --- p.18 / Chapter 1.11.2 --- Principles --- p.20 / Chapter 1.11.3 --- SAGE Application in Cancer and Immunology Studies --- p.22 / Chapter 1.11.4 --- Improvement of SAGE --- p.23 / Chapter 1.11.5 --- Bioinformatics --- p.24 / Chapter 1.12 --- DNA Microarray --- p.27 / Chapter 1.12.1 --- Introduction --- p.27 / Chapter 1.12.2 --- Application --- p.28 / Chapter 1.12.3 --- Method of cDNA Microarray --- p.28 / Chapter Chapter Two --- Serial Analysis of Gene Expression / Chapter 2.1 --- Introduction --- p.30 / Chapter 2.2 --- Material and Methods --- p.32 / Chapter 2.2.1 --- Mushroom cultivation and RNA extraction --- p.32 / Chapter 2.2.2 --- RNA Quality Estimation --- p.33 / Chapter 2.2.3 --- mRNA Isolation --- p.34 / Chapter 2.2.4 --- Serial Analysis of Gene Analysis (SAGE) --- p.34 / Chapter 2.2.4.1 --- Binding mRNA to magnetic beads for cDNA synthesis --- p.34 / Chapter 2.2.4.2 --- DNA synthesis verification --- p.35 / Chapter 2.2.4.3 --- NlaIII digestion --- p.36 / Chapter 2.2.4.4 --- NlaIII digestion verification --- p.36 / Chapter 2.2.4.5 --- Adapters ligation --- p.37 / Chapter 2.2.4.6 --- Cleaving with tagging enzyme --- p.37 / Chapter 2.2.4.7 --- Ditags creation --- p.38 / Chapter 2.2.4.8 --- PCR optimization and scale-up --- p.38 / Chapter 2.2.4.9 --- Polyacrylamide gel electrophoresis --- p.39 / Chapter 2.2.4.10 --- Eluting DNA from the gel --- p.40 / Chapter 2.2.4.11 --- NlaIII Cleavage and polyacrylamide gel electrophoresis --- p.40 / Chapter 2.2.4.12 --- Concatemers Ligation --- p.41 / Chapter 2.2.4.13 --- Cloning Concatemers into pZErO®-1 --- p.42 / Chapter 2.2.5 --- One Shot® TOP 10 Electrocomp´ёØ E. Coli transformation --- p.42 / Chapter 2.2.6 --- PCR Screening and Sequencing --- p.44 / Chapter 2.2.7 --- Sequence Analysis --- p.44 / Chapter 2.3 --- Results --- p.45 / Chapter 2.3.1 --- RNA Extraction --- p.45 / Chapter 2.3.2 --- cDNA Synthesis --- p.45 / Chapter 2.3.3 --- NlaIII digestion --- p.45 / Chapter 2.3.4 --- PCR amplification --- p.46 / Chapter 2.3.5 --- Gel-purification of the 100bp Ditags --- p.46 / Chapter 2.3.6 --- Isolation of the 26bp Ditags --- p.46 / Chapter 2.3.7 --- Concatemers Generation --- p.47 / Chapter 2.3.8 --- PCR Screening --- p.47 / Chapter 2.3.9 --- The Abundance and Identity of SAGE Tags --- p.48 / Chapter 2.4 --- Discussion --- p.86 / Chapter 2.4.1 --- RNA Extraction --- p.86 / Chapter 2.4.2 --- cDNA Synthesis and NlaIII digestion --- p.86 / Chapter 2.4.3 --- PCP amplification --- p.87 / Chapter 2.4.4 --- SAGE Tags Analysis --- p.88 / Chapter Chapter Three --- Microarray / Chapter 3.1 --- Introduction --- p.96 / Chapter 3.2 --- Materials and Methods --- p.97 / Chapter 3.2.1 --- Microarray chip preparation --- p.97 / Chapter 3.3.2 --- Sample preparation --- p.97 / Chapter 3.2.3 --- cDNA Synthesis and Sample labeling --- p.98 / Chapter 3.2.4 --- cDNA Purification --- p.99 / Chapter 3.2.5 --- cDNA analysis --- p.99 / Chapter 3.2.6 --- Array Hybridization --- p.102 / Chapter 3.2.6.1 --- Sample Preparation --- p.102 / Chapter 3.2.6.2 --- Hybridization Procedure --- p.102 / Chapter 3.2.7 --- Stringency Washes --- p.103 / Chapter 3.2.8 --- Detection with TSA --- p.103 / Chapter 3.2.9 --- Scanning and Analysis --- p.105 / Chapter 3.3 --- Results --- p.109 / Chapter 3.4 --- Discussion --- p.120 / Chapter Chapter Four --- Full Length Sequencing / Chapter 4.1 --- Introduction --- p.124 / Chapter 4.2 --- Material and Methods --- p.124 / Chapter 4.3 --- Results and Discussion --- p.125 / Chapter Chapter Five --- General Discussion --- p.149 / Appendix --- p.155 / References --- p.157
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324447 |
| Date | January 2003 |
| Contributors | Chum, Wing Yan., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 178 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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