Phytophthora root and
stem rot is the most destructive soybean soil-borne disease worldwide and can
be managed using soybean cultivars with genes conferring resistance to <i>Phytophthora
sojae</i>. Here we show that soybean <i>Rps11</i>
is an ~27-kb nucleotide-binding site leucine-rich repeat (NLR) gene that
confers broad-spectrum resistance to the pathogen. This giant gene is located in a genomic region
containing 12 unusually large NLR genes of a single origin and was formed by
rounds of intergenic/intragenic unequal recombination that involves the
promoter regions and the LRR regions. Comparison of the genomic region in the
Rps11 donor line with its corresponding regions in 29 diverse soybean genomes
revealed drastic regional diversification including NLR copy number variation
ranging from 5 to 23, and absence of allelic copy of <i>Rps11</i> in all 29
genomes. This study highlights innovative evolution and complexity of an NLR
cluster and enables precise selection of <i>Rps11</i> for cultivar improvement.
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/15074754 |
| Date | 29 July 2021 |
| Creators | Weidong Wang (11203863) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | In Copyright |
| Relation | https://figshare.com/articles/thesis/A_GIANT_CHIMERIC_NLR_GENE_CONFERS_BROAD_RESISTANCE_TO_PHYTOPHTHORA_ROOT_AND_STEM_ROT_OF_SOYBEAN/15074754 |
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