The Drosophila genome is thought to have five recA like proteins: Rad51B, Rad51C, Rad51D, XRCC2 and XRCC3. In Drosophila Rad51/SpnA, XRCC3/SpnB, and Rad51 C/SpnD participate in homologous recombination repair. The function of DmRad51 D and DmXRCC2 are unknown. The goal of this project was to elucidate the function ofXRCC2 in Drosophila. RNA interference allowed us to knockdown the function XRCC2 and its possible binding partner Rad51D. It was seen the knocking down the function of either XRCC2 or Rad51D does not affect the viability of the fly. However, drug treatment data does not allow us to make any conclusions about how the knockdown ofXRCC2 affects the viability of the fly. RNA in-situ hybridization shows highly intricate and complex branching patterns for XRCC2, which resembles the embryonic tracheal system. Lastly, XRCC2 was purified to generate an antibody made to recognize the XRCC2 protein will help localize the XRCC2 protein in future studies as well as determine protein-protein interactions with XRCC2.
| Identifer | oai:union.ndltd.org:pacific.edu/oai:scholarlycommons.pacific.edu:uop_etds-1746 |
| Date | 01 January 2010 |
| Creators | Montemayor, Phoebe E. |
| Publisher | Scholarly Commons |
| Source Sets | University of the Pacific |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | University of the Pacific Theses and Dissertations |
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