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Ganglioside Increases Metastatic Potential and Susceptibility of Prostate Cancer to Gene Therapy in vitro

Prostate cancer (CaP) is the 2nd most common cancer in North American men. Tumour management strategies are appropriate for early stage disease, but advanced disease has a poor prognosis and requires prompt treatment. Therefore, research into delay of tumour progression and efficacious treatment of aggressive cancer are of interest. Ganglioside was assessed for its role in altering markers of metastatic potential and susceptibility of CaP to adenovirus-mediated gene therapy. Healthy (RWPE-1) and malignant (DU-145, PC-3) prostate cells were cultured with or without mixed ganglioside. Differences in growth, ganglioside and integrin densities, and adenoviral infectivity were assessed between treatment and control groups. Ganglioside decreased (p<0.01) growth of PC-3 cells relative to untreated control. Ganglioside decreased (p<0.01) GD1a and increased (p<0.04) integrin densities in malignant prostate cells, suggesting ganglioside may increase metastatic potential of CaP. Ganglioside significantly increased adenovirus entry in PC-3 cells, thereby improving susceptibility of CaP to adenovirus-mediated gene therapy. / Nutrition and Metabolism

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/742
Date11 1900
CreatorsMiklavcic, John
ContributorsClandinin, M Tom (Agricultural, Food and Nutritional Science), Clandinin, M Tom (Agricultural, Food and Nutritional Science), Mazurak, Vera C (Agricultural, Food and Nutritional Science), Hitt, Mary M (Experimental Oncology)
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
Languageen_US
Detected LanguageEnglish
TypeThesis
Format508528 bytes, application/pdf
RelationMiklavcic, John (2009). http://www.asco.org/ASCOv2/Meetings/Abstracts?&vmview=abst_detail_view&confID=64&abstractID=20076, Miklavcic, John (2009). http://www.aocs.org/meetings/annual_mtg/index.cfm?page=am09_techprog.htm&interest=Health%20and%20Nutrition

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