Yue, Yanan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 62-63). / Abstract also in Chinese. / ABSTRACT (Chinese) --- p.i / ABSTRACT --- p.iii / CONTENT --- p.v / ACKNOWLEDGMENT --- p.vii / ABBREVIATIONS --- p.viii / Chapter CHAPTER 1 --- Introduction and Background / Chapter 1.1 --- Methods of Gene Delivery --- p.1 / Chapter 1.1.1 --- Viral Delivery Systems --- p.2 / Chapter 1.1.2 --- Non-Viral Delivery Systems --- p.3 / Chapter 1.2 --- The Gene-delivery Problems --- p.7 / Chapter 1.2.1 --- Extracellular Barriers --- p.8 / Chapter 1.2.2 --- Intracellular Barriers --- p.10 / Chapter 1.3 --- Polymer-Mediated Systems for Gene Delivery --- p.13 / Chapter 1.3.1 --- Polyethylenimine (PEI)-Based Vectors --- p.13 / Chapter 1.3.2 --- Cyclodextrin-Based Vectors --- p.15 / Chapter 1.4 --- Objective and Main Achievements --- p.16 / Chapter 1.5 --- References --- p.18 / Chapter CHAPTER 2 --- Effect of Free Polyethylenimine-Mediated Polycations on Gene Delivery: Fundamentals and Vital Factors / Chapter 2.1 --- Introduction --- p.24 / Chapter 2.2 --- Experimental Section --- p.25 / Chapter 2.3 --- Results and Discussions --- p.29 / Chapter 2.3.1 --- Fundamentals --- p.29 / Chapter 2.3.2 --- Vital Factors for the Efficacy of Free Chains --- p.37 / Chapter 2.4 --- Conclusions --- p.42 / Chapter 2.5 --- References --- p.42 / Chapter CHAPTER 3 --- Effect of Free Polyethylenimine-Mediated Polycations on Gene Delivery: Mechanistic Study / Chapter 3.1 --- Introduction --- p.44 / Chapter 3.2 --- Experimental Sections --- p.46 / Chapter 3.3 --- Results and Discussion / Chapter 3.3.1 --- Potential Effect of Free PEI Chains on Cellular Uptake --- p.49 / Chapter 3.3.2 --- Potential Effect of Free PEI Chains on Endolysosomal Release --- p.51 / Chapter 3.3.3 --- Exploration on Proton Sponge Hypothesis --- p.53 / Chapter 3.3.4 --- Interactions of PEI-based Polycations and Phospholipid Membranes --- p.55 / Chapter 3.4 --- Conclusions --- p.61 / Chapter 3.5 --- References --- p.62
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326856 |
| Date | January 2009 |
| Contributors | Yue, Yanan., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 63 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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