Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy

Description

Characterization of the RNA transcriptome by next-generation sequencing can produce an unprecedented yield of information that provides novel biologic insights. I describe four approaches for sequencing different aspects of the transcriptome and provide computational tools to analyze the resulting data. Methods that query the dynamic range of gene expression, low expressing transcripts, micro RNA levels, and start-site usage of transcripts are described.

Links & Downloads

1. Christodoulou, Danos C. 2013. Methods for comprehensive transcriptome analysis using next-generation sequencing and application in hypertrophic cardiomyopathy. Doctoral dissertation, Harvard University.
2. http://dissertations.umi.com/gsas.harvard:10749
3. http://nrs.harvard.edu/urn-3:HUL.InstRepos:11156672

Tags

Genetics

fhl1

genetic modifier

hypertrophic cardiomyopathy

inherited cardiomyopathies

next-generation sequencing

rna-seq

Additional Fields

Identifer	oai:union.ndltd.org:harvard.edu/oai:dash.harvard.edu:1/11156672
Date	08 October 2013
Creators	Christodoulou, Danos C.
Contributors	Seidman, Jonathan G., Seidman, Christine Edry
Publisher	Harvard University
Source Sets	Harvard University
Language	en_US
Detected Language	English
Type	Thesis or Dissertation
Rights	open