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Regulation of tubulin gene expression in sea urchin embryos

Regulation of tubulin gene expression in embryos of the sea urchin Lytechinus pictus has been experimentally investigated by use of cloned recombinant tubulin DNA and anti-tubulin antiserum. Tubulin synthesis appears to be autogenously regulated at the level of tubulin mRNA stability by the level of unpolymerized tubulin; i.e., the more unpolymerized tubulin, the less stable the tubulin mRNA. Destabilization of tubulin mRNA requires continued protein synthesis. Most of tubulin stored in eggs is unpolymerized; during embryogenesis the mass of tubulin per embryo changes little, but unpolymerized tubulin is increasingly polymerized into microtubules. There is a transcriptional stimulation of tubulin genes at the time of ciliogenesis but thereafter autoregulation by the ontogenetic decrease of the level of unpolymerized tubulin plays a predominant role for an increasing accumulation of tubulin mRNA. Deciliation results in a transient enhancement of transcription of tubulin genes, which is independent of the level of unpolymerized tubulin and does not require protein synthesis.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74267
Date January 1987
CreatorsGong, Zhiyuan.
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Biology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 000550045, proquestno: AAINN63436, Theses scanned by UMI/ProQuest.

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