"This thesis is a report on the feasibility of isolating a certain group of mutants. I call these mutants the "p.i.-related" mutants; an explanation of what they are and of my rule for grouping them together is given here. Bacterial chromosome replication consists of at least three steps: initiation, polymerization and termination/segregation (which may or may not comprise a single step). Inhibition of the polymerization step ("polymerization inhibition" or "p.i.") in a rapidly-growing bacterial population causes morphological distortion of the cells, a high death rate among them, and a number of other consequences. Recently, two great regulatory systems for bacterial cell behavior (described in Chapters IX and X) have been elucidated, and it appears that many of the consequences of p.i. might be explainable as the malfunctioning of these two systems. I define a "p.i.-related mutation" as a mutation that satisfies two criteria: First, it either causes p.i., mimics p.i., prevents p.i. or blocks the effects of p.i. Second, it exerts its effects by disrupting one of the two above-mentioned regulatory systems. The main question asked and answered in this report is: How good is each of three different methods of selection for p.i.-related mutants?"-- / Typescript. / "August, 1979." / "Submitted to the Department of Biological Science in partial fulfillment of the requirements for the degree of Master of Science." / Advisor: Johan H. Stuy, Professor Directing Thesis. / Includes bibliographical references (leaves 383-434).
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_223873 |
| Contributors | Graham, Geoffrey J. (author), Stuy, Johan H. (professor directing thesis), Florida State University (degree granting institution) |
| Publisher | Florida State University, Florida State University |
| Source Sets | Florida State University |
| Language | English, English |
| Detected Language | English |
| Type | Text, text |
| Format | 1 online resource (x, 435 leaves), online resource |
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