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Previous issue date: 2014-03-27 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / The African snail Achatina fulica Bowdich, 1822, is an invasive species that cause environmental and economic damage, as to human and animal health. The aim of this study was to evaluate the occurrence and parasitological aspects of A. fulica in Feira de Santana (BA). To assess the occurrence of A. fulica, sampling was made from January to June 2013 at four points in the urban perimeter of the city. In the laboratory animals were measured as the total length of the shell and the total mass. From these data the degree of development of the population was estimated, and the relationships between morphometric and climatic variables and the condition factor were calculated. During this period were collected 600 snails showing predominant length in the range of 57.0 to 47.6 mm. Results allowed to infer that the population of A. fulica in these sampling points is in decline, since in these areas were found juvenile and young animals. There was no association between climatic and morphometric variables. The relationship between total length and weight showed that A. fulica presented a negative allometric growth. The condition factor presented variations on time over the six months of collection. In order to evaluate parasitological aspects in A. fulica, animals were collected from may 2012-to june 2013 in eight areas in the urban perimeter of the city.The animals were collected early in the morning and transported to the laboratory of the Zoonoses and Public Health Research Group -UEFS. Each clam was analyzed individually using the artificial digestion technique in hydrochloric acid solution with identification of larvae found. The larvae have been identified as Metastrongylidae were submitted to the polymerase chain reaction for the identification of the genus Angyostrongylus. 220 animals were tested and in 28 (12.7; 95: 8.6 -17.9) were found at least one of four morphotypes of nematodes. Angyostrongylus has not been registered in snails examined. There was no association between climatic variables and the presence of larvae. The location of collection and the size of the snail influenced in the larvae of nematodes. Both parasited and not parasitised animals have negative allometric growth. The relative condition factor did not differ significantly between the groups. Nested-PCR has been used to search Cryptosporidium ssp and Sarcocystidae DNA in fecal samples of Achatina fulica. Amplicons compatibles with Cryptosporidium ssp were found in 2 of 223 stool samples analyzed. Of the 223 stool samples analysed, in 58 samples we found the 290 base pair fragment expected for Sarcocystidae, and then subjected to RFLP. Of these, 41 samples were cleaved by enzymes used in this study, producing a profile compatible with T. gondii. The other 17 samples were not compatible with Sarcocystidae. This is the first record of the occurrence of A. fulica in Feira de Santana (BA), with research of association of climatic variables to the biometric parameters of the animals. These results indicate the presence of animal and humana parasites in Achatina fulica in the municipality of Feira de Santana, demonstrating the potential of these animals in the maintenance and spread of these agents in the environment. / O caramujo africano Achatina fulica Bowdich, 1822, ? uma esp?cie invasora que causa preju?zos ambientais, econ?micos e a sa?de humana e animal. O objetivo desse estudo foi avaliar a ocorr?ncia e os aspectos parasit?rios de Achatina fulica no munic?pio de Feira de Santana (BA). Para avalia??o da ocorr?ncia de A. fulica foram feitas coletas de janeiro a junho de 2013 em quatro pontos no per?metro urbano do munic?pio. No laborat?rio os animais foram mensurados quanto ao comprimento total da concha e a massa total. A partir desses dados o grau de desenvolvimento da popula??o foi estimado, as rela??es entre as vari?veis morfom?tricas e clim?ticas e o fator de condi??o foram calculados. Durante esse per?odo foram coletados 600 caramujos apresentando comprimento predominante no intervalo de 47,6-57,0mm. Os resultados permitiram inferir que a popula??o de A. fulica nos pontos de coleta estudados est? em decl?nio, j? que nessas ?reas foram encontrados animais juvenis e jovens. N?o houve associa??o entre as vari?veis clim?ticas e morfom?tricas. A rela??o entre o comprimento total e a massa corporal mostrou que A. fulica apresentou um crescimento alom?trico negativo. O fator de condi??o apresentou varia??es pontuais ao longo dos seis meses de coleta. A fim de avaliar os aspectos parasit?rios em A. fulica foram realizadas coletas de maio de 2012 a junho de 2013 em oito ?reas no per?metro urbano do munic?pio, com registro de observa??es sobre os locais de coleta. Cada molusco foi analisado individualmente utilizando a t?cnica de digest?o artificial em solu??o de ?cido clor?drico, com identifica??o das larvas encontradas. As larvas identificadas como Metastrongylidae foram submetidas ? rea??o em cadeia pela polimerase para a identifica??o do g?nero Angyostrongylus. Foram analisados 220 animais e em 28 (12,7%; IC95%: 8,6-17,9) foram encontrados pelo menos um de quatro morfotipos de nemat?deos. Angyostrongylus n?o foi registrado nos caramujos examinados. N?o houve associa??o entre vari?veis clim?ticas e a presen?a de larvas. O local de coleta e o tamanho do caramujo influenciaram no encontro de larvas de nemat?deos. Tanto animais parasitados quanto n?o parasitados apresentaram um crescimento alom?trico negativo. O fator de condi??o relativo n?o diferiu significamente entre os grupos. A fim de pesquisar a presen?a de DNA Cryptosporidium ssp. e Sarcocystidae nas amostras fecais de Achatina fulica foi utilizada a t?cnica de nested-PCR (nPCR). Para Cryptosporidium ssp., amplicons compat?veis com este parasito foram encontrados em 2 de 223 amostras de fezes analisadas. Das 223 amostras de fezes de Achatina fulica submetidas ? nPCR para detec??o de Sarcocystidae, 58 amostras amplificaram o fragmento esperado de 290 pares de base, sendo ent?o submetidas ? RFLP. Destas, 41 amostras foram clivadas pelas enzimas utilizadas nesse estudo, produzindo um perfil compat?vel com T. gondii. As outras 17 amostras n?o foram compat?veis com o Sarcocystidae. Este ? o primeiro registro da ocorr?ncia de A. fulica no munic?pio de Feira de Santana (BA), com pesquisa da associa??o das vari?veis clim?ticas aos par?metros biom?tricos dos animais estudados. Estes resultados indicam que h? presen?a de parasitos de interesse em sa?de animal e humana em Achatina fulica no munic?pio de Feira de Santana, Bahia, demonstrando o potencial destes animais na manuten??o e dispers?o destes agentes no ambiente.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/134 |
| Date | 27 March 2014 |
| Creators | Almeida, Patr?cia Herc?lia Arcanjo de |
| Contributors | Silva, Aristeu Vieira da |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado em Zoologia, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7322093382187536113, 600, 600, 600, 600, -6971480722008537872, -6482652380601267558, 4767858349021390776 |
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