"January 2003" / Addendum on back page. / Bibliography: p. 175-184. / 184 p. : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / "In the study reported in this thesis, a PCR-based approach was used to isolate the b-like globin genes that are present in the tammar wallaby, Macropus eugenii, including the gene that encodes the w-globin chain. Three -like globin genes (b-, e-, w-) that had previously been described at the protein level in the tammar wallaby were characterised. w-globin orthologues were also identified in a wide range of marsupial species, and in one of these species, the dunnart (Sminthopsis crassicaudata), the complete DNA sequence of the w-globin gene was determined. Southern analysis in the dunnart and in situ hybridisation in the tammar wallaby, provided evidence for the unexpected conclusion that w-globin is not part of the -globin gene cluster in these species. RT-PCR studies using RNA isolated from a new-born dunnarts confirmed that w-globin is expressed in this species. Therefore, this is the first report of an "orphaned" b-like globin gene that is expressed in a vertebrate." --p. 6. / Thesis (Ph.D.)--University of Adelaide, Dept. of Molecular Biosciences, 2003
| Identifer | oai:union.ndltd.org:ADTP/263176 |
| Date | January 2003 |
| Creators | Wheeler, David William |
| Source Sets | Australiasian Digital Theses Program |
| Language | en_US |
| Detected Language | English |
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