Bacteria of the genus Xenorhabdus live as the natural symbionts of the entomopathogenic nematodes of the family Steinernematidae. They produce a wide range of toxic secondary metabolites of different chemical structure and effect. The synthesis of particular products is specific for each strain of Xenorhabdus. The thesis is focused on evaluating the impact of bacterial metabolites on facultatively entomoparasitic nematodes Oscheius myriophila, the free-living nematode Caenorhabditis elegans and the phytophagous potato cyst nematode Globodera rostochiensis. Target species of nematodes were exposed to 37 strains of Xenorhabdus bacteria, isolated from various species of Steinernematidae. Testing the impact of bacterial metabolites on O. myriophila and C. elegans nematodes was performed both by direct cultivation of target species on solid medium with live bacterial cultures as well as in sterilized liquid bacteria cultures. The effect of toxic substances on G. rostochiensis was evaluated only in selected sterilized bacteria cultures.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:395267 |
| Date | January 2019 |
| Creators | JAKUBÍKOVÁ, Hedvika |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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