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Karakteristike gluteninsko-glijadinskog kompleksa zrna kao pokazatelj tehnološkog kvaliteta pšenice / Characteristics of glutenin-gliadin complex as aparameter of wheat technological quality

<p>U radu je ispitivan uticaj karakteristika glutenina i<br />glijadina uzoraka p&scaron;enice domaćeg sortimenta kako<br />bi se na osnovu tumačenja njihove strukture<br />omogućilo dono&scaron;enje egzaktnih zaključaka o<br />uzročno-posledičnom odnosu vezanom za stanje<br />gluteninsko-glijadinskog kompleksa zrna različitih<br />sorti p&scaron;enice i njihovog ispoljenog tehnolo&scaron;kog<br />kvaliteta posmatrano i u zavisnosti od uticaja<br />spoljne sredine. Za ispitivanje su kori&scaron;ćene A-PAGE<br />i SDS-PAGE elektroforetske tehnike ispitivanja<br />proteinskih frakcija, uz pomoć profesionalnog<br />softverskog paketa za obradu elektroforegrama.<br />Takođe, kori&scaron;ćene su i standardne metode za<br />ispitivanje tehnolo&scaron;kog kvaliteta p&scaron;enice i njihove<br />modifikacije. Na osnovu dobijenih rezultata<br />utvrđeno je da je tehnolo&scaron;ki kvalitet p&scaron;enice<br />neophodno povezivati i sa gluteninskim i sa<br />glijadinskim frakcijama proteina i da su osobine<br />otpornosti prema proteolitičkim procesima i<br />temperaturnom stresu sortno specifične. Takođe je<br />utvrđeno da su elektroforetske tehnike primenljive<br />u tumačenju tehnolo&scaron;kog kvaliteta p&scaron;enice, a da su<br />modifikacije standardnih metoda pokazale prednost<br />u objektivnijem i bržem predviđanju tehnolo&scaron;kog<br />kvaliteta domaćeg sortimenta hlebnih p&scaron;enica u<br />odnosu na standardne metode ispitivanja<br />tehnolo&scaron;kog kvaliteta p&scaron;enice.</p> / <p>The influence of glutenin and gliadin characteristics of<br />samples of domestic wheat varieties was investigated<br />with the aim to obtain on the basis of their structure,<br />conclusions about the interactions of condition of<br />glutenin and gliadin complex of the kernel of different<br />wheat varieties and their expressed technological<br />quality in the dependence of agro ecological growing<br />conditions. A-PAGE and SDS-PAGE electrophoretic<br />techniques were used for the investigation, with<br />consequent use of professional software for<br />electrophoregrams analysis. Standard and modified<br />methods were used for determination of wheat<br />technological quality. On the basis of obtained results<br />it was concluded that the technological quality of<br />wheat should be analyzed based structure of both<br />glutenin and gliadin fractions of wheat proteins, and<br />that the resistance to both, temperature stress and<br />proteolitic degradation is variety specific.<br />Electrophoretic techniques are applicable in analysis<br />of wheat technological quality. Modification of<br />standard methods expressed advantages in more<br />objective and more rapid determination and<br />foreseeing of technological quality of domestic wheat<br />varieties in comparison to standard methods.</p>

Identiferoai:union.ndltd.org:uns.ac.rs/oai:CRISUNS:(BISIS)71282
Date02 November 2007
CreatorsTorbica Aleksandra
ContributorsAntov Mirjana, Mastilović Jasna, Knežević Desimir
PublisherUniverzitet u Novom Sadu, Tehnološki fakultet Novi Sad, University of Novi Sad, Faculty of Technology at Novi Sad
Source SetsUniversity of Novi Sad
LanguageSerbian
Detected LanguageEnglish
TypePhD thesis

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