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Automated quantitative method for urinary mucopolysaccharides.

by Ngai Yuk Keung. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 69-75). / SUMMARY --- p.1 / Chapter 1. --- INTRODUCTION --- p.3 / Chapter 2. --- LITERATURE REVIEW --- p.4 / Chapter 2.1 --- Properties of GAGs --- p.4 / Chapter 2.1.1 --- FUNCTION OF GAGs --- p.6 / Chapter 2.1.2 --- METABOLISM OF GAGs --- p.6 / Chapter 2.2 --- Mucopolysaccharidoses --- p.12 / Chapter 2.2.1 --- CLINICAL PRESENTATION AND GENETICS OF MPS --- p.13 / Chapter 2.2.2 --- Treatment --- p.13 / Chapter 2.3 --- Laboratory tests for the diagnosis of MPS --- p.17 / Chapter 2.3.1 --- SCREENING --- p.17 / Chapter 2.4 --- Confirmation method --- p.20 / Chapter 2.4.1 --- Enzyme assay --- p.20 / Chapter 2.4.2 --- Electrophoresis --- p.21 / Chapter 2.4.3 --- Chromatography --- p.22 / Chapter 2.5 --- Conclusion of Literature Review --- p.23 / Chapter 2.6 --- Choice of method --- p.24 / Chapter 2.7 --- The present study --- p.25 / Chapter 3. --- MATERIALS --- p.26 / Chapter 3.1 --- Material --- p.26 / Chapter 3.2 --- Stock solution --- p.26 / Chapter 3.3 --- Stock standards --- p.26 / Chapter 3.4 --- Working standards --- p.27 / Chapter 3.5 --- Working DMB solution --- p.27 / Chapter 3.6 --- Instruments --- p.27 / Chapter 3.7 --- Statistics --- p.27 / Chapter 3.8 --- Urine samples --- p.28 / Chapter 4. --- METHODS --- p.29 / Chapter 4.1 --- Studies on spectral characteristics --- p.29 / Chapter 4.1.1 --- Spectrum of DMB --- p.29 / Chapter 4.1.2 --- Spectra of DMB-GAG products --- p.29 / Chapter 4.2 --- Studies on stability --- p.29 / Chapter 4.2.1 --- Stability of DMB dye in assay condition --- p.29 / Chapter 4.2.2 --- Stability of DMB-GAG products in assay condition --- p.30 / Chapter 4.3 --- Linearity studies --- p.30 / Chapter 4.3.1 --- Linearity of DMB dye --- p.30 / Chapter 4.3.2 --- Linearity of GA G assay --- p.32 / Chapter 4.4 --- Detection limit --- p.32 / Chapter 4.5 --- Precision study --- p.32 / Chapter 4.5.1 --- Within-run precision --- p.32 / Chapter 4.5.2 --- Between-day precision --- p.32 / Chapter 4.6 --- Interference study --- p.33 / Chapter 4.6.1 --- Haemoglobin --- p.33 / Chapter 4.6.2 --- Protein --- p.33 / Chapter 4.7 --- Recovery study --- p.34 / Chapter 4.8 --- Correlation study --- p.34 / Chapter 4.9 --- Automated measurement of urinary GAG --- p.35 / Chapter 4.10 --- Reference range --- p.35 / Chapter 4.11 --- GAG in mentally retarded patients --- p.36 / Chapter 5. --- RESULTS --- p.37 / Chapter 5.1 --- Studies on spectral Characteristics --- p.37 / Chapter 5.1.1 --- DMB Spectrum --- p.37 / Chapter 5.1.2 --- Spectra of DMB-GAG products --- p.37 / Chapter 5.2 --- Studies on stability --- p.43 / Chapter 5.2.1 --- Stability of DMB dye in assay condition --- p.43 / Chapter 5.2.2 --- Stability ofDMB-GAG reaction mixture in assay condition --- p.43 / Chapter 5.3 --- Linearity studies --- p.43 / Chapter 5.3.1 --- Linearity of DMB dye --- p.43 / Chapter 5.3.2 --- Linearity of GAG assay --- p.47 / Chapter 5.3.3 --- Detection limit --- p.50 / Chapter 5.4 --- Precision study --- p.50 / Chapter 5.5 --- Interference study --- p.52 / Chapter 5.5.1 --- Haemoglobin --- p.52 / Chapter 5.5.2 --- Protein --- p.52 / Chapter 5.6 --- Recovery study --- p.52 / Chapter 5.7 --- Correlation study --- p.52 / Chapter 5.8 --- Reference range --- p.55 / Chapter 5.9 --- GAG in mentally retarded patients --- p.55 / Chapter 6. --- DISCUSSION --- p.60 / Chapter 6.1 --- Analytical Performance --- p.62 / Chapter 6.2 --- Clinical performance --- p.64 / Chapter 6.2.1 --- Reference population --- p.64 / Chapter 6.2.2 --- Mentally retarded patients --- p.65 / Chapter 6.2.3 --- Suggestion for future studies --- p.66 / Chapter 7. --- CONCLUSION --- p.67 / Chapter 8. --- APPENDIX --- p.68 / Chapter 9. --- REFERENCE --- p.69

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_321486
Date January 1996
ContributorsNgai, Yuk Keung., Chinese University of Hong Kong Graduate School. Division of Clinical Biochemistry.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, 75 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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