The glyoxalase system, which has been known since 1913, is widely distributed in nature. The system consists of two enzymes, glyoxalase I and glyoxalase II. Methylglyoxal is very unstable and undergoes oxidation and polymerization reactions. One of the purposes of this study was to find a simple, convenient and reproducible method of methylglyoxal preparation. Another objective was the purification of both glyoxalase enzymes employing affinity chromatography as a major step. The purified enzymes were to be characterized by chemical, physical and kinetic properties as an approach to the understanding of the biological function of the system.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc330887 |
| Date | 12 1900 |
| Creators | Oray, Bedii |
| Contributors | Norton, S. J., Jacobson, Myron, Russell, Benny, Skinner, Charles Gordon, Brady, William Thomas |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | ix, 110 leaves : ill., Text |
| Rights | Public, Oray, Bedii, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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