Thesis (PhD (Biochemistry))--Stellenbosch University, 2008. / This study describes:
• the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20
lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) and
cytochrome b5 from the South African Angora goat;
• the identification of two CYP17 genes encoding two unique CYP17
isoforms in the Angora goat;
• the development of a UPLC-APCI-LC method for the separation and
quantification of seven adrenal steroids;
• the characterisation of the enzymatic activity of the two Angora CYP17
isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvalues
for the metabolism of pregnenolone and progesterone were
determined;
• the development of a rapid and accurate real-time PCR genotyping test for
CYP17 in Angora goats. Three unique genotypes were identified;
• the determination of blood cortisol levels upon the stimulation of the HPAaxis
by intravenous insulin injection in the three Angora goat genotypes.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/1101 |
| Date | 12 1900 |
| Creators | Storbeck, Karl-Heinz |
| Contributors | Swart, P., Swart, A. C., Stellenbosch University. Faculty of Science. Dept. of Biochemistry. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | Stellenbosch University |
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