An appropriate selection of isolated oocytes with respect to the level of folliculogenesis is one of the ways to improve current methods. For this reason, this disertation thesis was focused on research of changes in distribution of cortical granules in different subpopulations of porcine oocytes with different meiotic competence during folliculogenesis. Oocytes from small (to less than or equal to 5mm) and medium (5-9 mm) follicles, collected from selected ovaries divided according to the phase of estral cycle, were used for the experiments. The oocytes were stained with PNA conjugated with fluorescent probe fluorescein isothiocyanate. A laser confocal microscope was used for visualization of the cortical granules. The experiment revealed a significant decrease of cortical granules in peripheral region of the ooplasm with increasing meiotic competence of oocytes during maturation in vitro. It also showed that oocytes with different meiotic competence differ in the ability to undergo normal fertilization and that fertilization in vitro in oocytes with lower meiotic competence is associated to higher incidence of polyspermic oocytes. However, disruption during cortical reaction as the cause for higher incidence of polyspermy could not be confirmed.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:425067 |
| Date | January 2016 |
| Creators | Hanuláková, Šárka |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.0016 seconds