Electrochemiluminescence (ECL) is a phenomenon whereby electrochemical reactions generate a product that is capable of emitting light. ECL’s high sensitivity, selectivity, extremely low background, and relatively simple instrumentation make it particularly well-suited for chemical sensing and biosensing strategies. Here we report a simple ECL imaging system based on a camera interfaced with a zoom lens to compare pencil graphite electrode (PGE) and screen-printed carbon electrode (SPCE) arrays as ECL platforms. With this system, ECL signals generated from tris(2,2′- bipyridine)ruthenium(II) chloride hexahydrate ([Ru(bpy)3]2+) using co-reactant tri-n-propylamine (TPA) were linear with respect to [Ru(bpy)3]2+ concentrations from 9 to 450 μM. Detection limits for [Ru(bpy)3]2+ were found to be 1.8 μM with PGEs and 0.9 μM with SPCEs. Immobilization of a thin polyvinylpyridine (PVP) film ECL reporter [Ru(bpy)2(PVP)10]2+ on SPCEs was also investigated. Overall, the combination of PGEs or SPCEs with the simple ECL imaging system offers a cost-effective approach to ECL-based sensing and biosensing.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-5873 |
| Date | 01 May 2024 |
| Creators | Ehigiator, Sandra |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations |
| Rights | Copyright by Sandra Omono Ehigiator |
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