This thesis studies the problematic of the new polymorphism APOA2 gene and that association with fatty acids contain in a group of Czech Large White pigs. APOA2 gene (ID: 100153243) is a candidate gene for porcine meat quality. The aim of thesis was to analyze the influence of selected polymorphisms on fatty acids and intramuscular fat contain. Among fatty acids was observed a contain of: tetradecenoic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, arachidic acid a eicosapentaenoic acid (EPA). Have been used molecular-genetic methods such as primers design in the OLIGO software, PCR, gel electrophoresis a sequencing by Sanger method. The results were processed by form of genotype frequency and followed by associative analysis with a mixed linear model. The values of the relative alleles frequency of polymorphism APOA2 T>A rs80803879 were as follows: A = 0,086, T = 0,914 and relative alleles frequency of APOA2 G>A rs331415849: A = 0,068 a G = 0,948. Polymorphism associations were found for fatty acids: myristoleic acid, acid palmitoleic acid, oleic acid, arachidonic acid, and arachidic acid.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:427182 |
| Date | January 2018 |
| Creators | Sukhov, Oleg |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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