In this study, biochemical techniques were used with immunological techniques to dissect the specific T cell reactivities to the PG molecule. Two T cell lines and two T cell hybridomas that belong to the T helper subset TH1 were isolated. The line JY.A recognized only the human fetal PG while the other line (JY.D) and the two T cell hybridomas (TH5 and TH14) recognized PG of all the different origins tested, except the rat chondrosarcoma and the mouse PG. The three latter T cells were found to recognize immunodominant T cell epitopes on the G1 domain of PG and cross-reacted with link protein. The presence of KS chain(s) close to T cell epitopes restricted the reactivity of the two T cell hybridomas. The location of one T cell hybridoma epitope (TH5) was contained within a peptide of twenty eight amino acids in the B region of G1. T cell line JY.A can induce early pathological change characteristic of rheumatoid arthritis when injected in the knee joint of naive BALB/c mice.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41140 |
| Date | January 1993 |
| Creators | Leroux, Jean-Yves |
| Contributors | Poole, A. Robin (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001327638, proquestno: NN87901, Theses scanned by UMI/ProQuest. |
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