Organ specific neoantigen (OSN) is shed from cancer of patients with metastatic lung cancer and is filtered into the urine. The OSN was purified and characterized by physicochemical methods. OSN activity was detected by the blocking of the tube LAI assay. Two different methods are described for isolating the OSN. By both procedures material was isolated that consisted of three to four polypeptide chains in the molecular weight range of 43,000 to 31,000 dalton and that had high OSN activity. Urinary protein isolated from normal subjects by the same methods did not have OSN activity and also lacked the 31,000 mol. wt. protein. Immunoprecipitation studies with specific antisera did not show the presence of (beta)(,2) microglobulin or HLA molecules in the OSN material. Furthermore, lung tumor associated antigens such as carcinoembryonic antigen, lactoferrin and (alpha)(,1) antichymotrypsin were absent from the final isolate. / Material containing the lung tumor OSN is glycosilated and has a pI of 5.0 to 6.0. By two dimensional gel electrophoresis the OSN material was heterogeneous showing many spots from a pH of 6.2 to 7.2 but all had a molecular weight of 31,000 dalton.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68651 |
| Date | January 1982 |
| Creators | Fink, Aaron. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Medicine) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000157533, proquestno: AAINK60944, Theses scanned by UMI/ProQuest. |
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