Medium chain fatty acid oxidation was investigated in riboflavin deficient and 2-octynoic acid treated rats. Urinary metabolites of deuterium labelled fatty acid substrates were determined using gas chromatography-mass spectrometry. Control and experimental animals both produced labelled dicarboxylic acids. The ratio of chain length of dicarboxylates (C$ sb{ rm n+2}$/C$ sb{ rm c}$) was lowest in riboflavin deficient rats. As mitochondrial $ beta$-oxidation is inhibited, Peroxisomal $ beta$-oxidation appears to increase in response to inhibited mitochondrial oxidation in riboflavin deficiency. Administration of L-carnitine with the labelled substrates to half the rats showed that there were no differences in organic acid excretion between carnitine supplemented and unsupplemented rats. A new method to quantitate individual acylcarnitines in urine using fast atom bombardment-mass spectrometry and deuterium labelled acylcarnitines as internal standards demonstrated that administration of L-carnitine did enhance excretion of free carnitine and short chain acylcarnitines along with some dicarboxylcarnitines in all groups.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75845 |
| Date | January 1988 |
| Creators | Montgomery, Jane Aimée. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000720271, proquestno: AAINL48531, Theses scanned by UMI/ProQuest. |
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