Hippi, HIP-1 Protein Interactor, is a novel protein that mediates the cellular toxicity of the Huntingtin Interacting Protein-1 (HIP-1). In order to establish a role for this protein in the pathogenesis of Huntington's Disease, targeted deletion of the Hippi locus in mice will be accomplished. First the murine counterpart to the human Hippi gene was cloned from various cDNA library sources. Secondly, the genomic structure of the Hippi gene was characterized in multiple mouse strains. Thirdly, using these Hippi genomic sequences, the gene targeting strategy was developed. Finally, the required knock-out vectors for Hippi deletion were constructed.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.33408 |
| Date | January 2000 |
| Creators | Houtzager, Vicky M. |
| Contributors | Nicholson, D. W. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biochemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001771621, proquestno: MQ70716, Theses scanned by UMI/ProQuest. |
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