<p> Full length P1 eDNA clones have been obtained from human
and CHO sources (Jindal et al., 1989; Picketts et al., 1989) which contain sequences that show extensive sequence and structural similarity to the chaperonin family of proteins,
including the mitochondrial hsp60 protein. In the studies described here human genomic DNA libraries were screened with human P1 (hsp60) eDNA probes and those clones containing P1
related sequences were isolated. One clone, pGem1b, was found to be completely homologous to the human P1 cDNA in both coding and non-coding regions, devoid of intervening sequences, and terminates at a point 24 base pairs upstream of the translation initiation signal (ATG). The other human clones analyzed were all found to be pseudogenes containing numerous additions, deletions and base substitutions, but no introns. A total of six different classes of pseudogenes were identified. Four of these were sequenced completely across the translated region of the functional P1 gene. Sequence homologues of 86.1, 87.4 89.7 and 90.2% were observed. </p> <p> In addition, rat kidney and mouse JTJ cell eDNA libraries were screened similarly for P1 sequences. The rat P1 eDNA sequence was obtained by combining the sequence information from three different clones. The clones obtained lacked the 5'- leader sequence as well as the mitochondrial targeting
sequence. However, the entire coding sequence for a mature P1 protein of 547 amino acids could be deduced. The mouse P1 DNA sequence was also obtained from three different clones. These clones contained a portion of the mitochondrial targeting sequence and the entire sequence for the mature P1 protein. The protein sequences of the rat and mouse P1 clones were highly homologous (98-99%) to those obtained from human and CHO sources. The calculated molecular weights of the mature rat and mouse P1 proteins are 57,916 and 57,940 daltons, respectively, which are in close agreement with those predicted for the human (57, 939 daltons) and CHO (57, 949) proteins (Jindal et al. 1 1989; Picketts et al., 1989). </p> / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/22479 |
| Date | 12 1900 |
| Creators | Venner, Thomas |
| Contributors | Gupta, R. S., Biochemistry |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
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