The measurement of the equilibrium constant and the thermodynamic parameters of the binding of hydroxide ions to the active site of hemerythrin, an invertebrate iron containing protein, was done by a new difference spectroscopic method. The method enables accurate measurements of equilibrium constants without accurate knowledge of molar absorptivities or protein concentrations and is novel in that it takes the difference between the two equilibrium mixtures, thus enabling the measurement of equilibrium constants when it is not convenient or possible to produce either substance as a pure reference material.
| Identifer | oai:union.ndltd.org:pdx.edu/oai:pdxscholar.library.pdx.edu:open_access_etds-4220 |
| Date | 01 January 1982 |
| Creators | McCallum, John David |
| Publisher | PDXScholar |
| Source Sets | Portland State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Dissertations and Theses |
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