by Ka-lun So. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 128-134. / CONTENT --- p.i / ACKNOWLEDGEMENTS --- p.v / ABBREVIATIONS --- p.vi / ABSTRACT --- p.vii / Chapter CHAPTER I --- LITERATURE REVIEW / Chapter 1.1 --- HALOBACTERIA AND THEIR GENOME STRUCTURES --- p.1 / Chapter 1.1.1 --- Halobacteria / Chapter 1.1.2 --- "Chromosome, satellite DNA & repeated sequences" / Chapter 1.1.3 --- Plasmids / Chapter 1.2 --- HALOPHAGES --- p.7 / Chapter 1.2.1 --- Persistent bacteriophage φP / Chapter 1.2.2 --- Phage φN / Chapter 1.2.3 --- Halophage φH / Chapter 1.2.4 --- Halophage Hsl / Chapter 1.2.5 --- Other halophages / Chapter 1.2.6 --- Halophages and persistent infection / Chapter 1.3 --- SUSCEPTIBILITY OF HALOBACTERIA TO ANTI-MICROBIAL AGENTS --- p.14 / Chapter 1.3.1 --- Antibiotics / Chapter 1.3.2 --- Heavy metals / Chapter 1.4 --- AIMS OF THIS STUDY --- p.17 / Chapter CHAPTER II --- MOLECULAR CHARACTERIZATION AND RESTRICTION MAPPING OF GENOME OF HALOPHAGE φP / Chapter 2.1 --- INTRODUCTION --- p.19 / Chapter 2.2 --- MATERIALS AND METHODS --- p.19 / Chapter 2.2.1 --- Production of high titer phage lysate / Chapter 2.2.2 --- Phage genomic DNA isolation and purification / Chapter 2.2.3 --- DNase and RNase treatment / Chapter 2.2.4 --- Agarose gel electrophoresis / Chapter 2.2.5 --- Nuclease Bal31 / Chapter 2.2.6 --- Restriction enzyme digestions / Chapter 2.2.7 --- Nick translation / Chapter 2.2.8 --- End-labelling / Chapter 2.2.9 --- Single and double enzyme digestions / Chapter 2.2.10 --- Non-denaturing polyacrylamide gel electrophoresis of DNA / Chapter 2.3 --- RESULTS AND DISCUSSIONS --- p.30 / Chapter CHAPTER III --- GENOMIC LIBRARY CONSTRUCTION AND SUPERCOILED DNA SEQUENCING OF φP DNA / Chapter 3.1 --- INTRODUCTION --- p.46 / Chapter 3.2 --- MATERIALS AND METHODS --- p.48 / Chapter 3.2.1 --- Restriction enzyme digestions / Chapter 3.2.2 --- Dephosphorylation / Chapter 3.2.3 --- Electro-elution / Chapter 3.2.4 --- Ligation / Chapter 3.2.5 --- Transformation / Chapter 3.2.6 --- Mini-preparation of plasmid / Chapter 3.2.7 --- Putative clone confirmation / Chapter 3.2.8 --- Plasmid preparation by QIAGEN column / Chapter 3.2.9 --- Supercoiled DNA sequencing / Chapter 3.2.10 --- DNA sequencing electrophoresis / Chapter 3.3 --- RESULTS AND DISCUSSIONS --- p.60 / Chapter CHAPTER IV --- PERSISTENT INFECTION OF HALOPHAGE φP / Chapter 4.1 --- INTRODUCTION --- p.72 / Chapter 4.2 --- MATERIALS AND METHODS --- p.73 / Chapter 4.2.1 --- Plaque forming assay / Chapter 4.2.2 --- Isolation of phage-releasing bacterial strains / Chapter 4.2.3 --- Bacteria growth kinetics measurement / Chapter 4.2.4 --- Analysis of supernatant of bacterial culture / Chapter 4.2.5 --- Analysis of cell pellet of bacterial culture / Chapter 4.2.6 --- Preparation and analysis of plasmids within halobacteria / Chapter 4.2.7 --- Analysis of the infection of halophage φP / Chapter 4.2.8 --- DNA hybridization / Chapter 4.2.9 --- Denaturing (SDS) discontinuous polyacrylamide gel electrophoresis / Chapter 4.3 --- RESULTS AND DISCUSSIONS --- p.84 / Chapter CHAPTER V --- DRUG SUSCEPTIBILITY OF Halobacterium cutirubrum / Chapter 5.1 --- INTRODUCTION --- p.98 / Chapter 5.2 --- MATERIALS AND METHODS --- p.98 / Chapter 5.2.1 --- 5-bromouridine susceptibility / Chapter 5.2.2 --- Ethidium bromide susceptibility / Chapter 5.2.3 --- Heavy metals susceptibility / Chapter 5.2.4 --- Antibiotics susceptibility / Chapter 5.2.5 --- Isolation of antibiotics resistant bacteria / Chapter 5.2.6 --- Total cellular DNAs isolation and analysis / Chapter 5.2.7 --- Cellular protein isolation and analysis / Chapter 5.3 --- RESULTS AND DISCUSSIONS --- p.103 / Chapter CHAPTER VI --- GENERAL DISCUSSIONS AND FURTHER STUDIES --- p.119 / REFERENCES --- p.128
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318576 |
| Date | January 1990 |
| Contributors | So, Ka-lun., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, viii, 134 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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