The extreme susceptibility to infection with Salmonella Typhimurium of wild-derived MOLF/Ei has been linked to one chromosomal region (Ity3). Toll-like receptor 5 (Tlr5) is located within the Ity3 interval and its candidacy as a Salmonella-susceptibility gene was evaluated in vitro and in vivo by comparing the Tlr5 C57BL/6J and MOLF/Ei alleles (85). In vitro studies using NF-kappaB-dependent reporter genes showed that sequence variants found within the coding region of MOLF/Ei Tlr5 did not affect the response of Tlr5 to flagellin compared to C57BL/6J. MOLF/Ei promoter had a slightly stronger basal activity than the C57BL/6J allele. In vivo study using Ity3 congenic mice showed that mice homozygous for MOLF/Ei allele at Ity3 had a stronger response to flagellin as measured by IL-6 secretion in the serum. Finally, we can conclude that Tlr5 is involved in the disease phenotype underlying Ity3 however, it is not clear if the impact of Tlr5 is primary or secondary.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.81587 |
| Date | January 2004 |
| Creators | Angers, Isabelle |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Human Genetics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002198638, proquestno: AAIMR12390, Theses scanned by UMI/ProQuest. |
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