Two RNA trafficking cis sequences (A2RE-1 and A2RE-2) were identified in HIV-1 RNA. Their activity is assessed here during HIV-1 gene expression. Single point mutations in each A2RE reduced the levels of bound hnRNP A2, but also resulted in a marked accumulation of viral genomic RNA in the nucleus and a significant reduction in genomic RNA encapsidation in progeny virions, with the strongest phenotype observed for the A2RE-2 mutant. Immunofluorescence analyses revealed marked changes in viral gene expression patterns for pr55Gae and Vpr, as well as a significant reduction in Vpr incorporation levels. Viral infections were markedly compromised in the A2RE-2 mutant but this virus quickly reverted in culture. These data point to the importance of the HIV-1 A2RE determinants and the trans factor hnRNP A2 in the control HIV-1 gene expression patterns. This also provides further details on the implication of host factors in RNA trafficking during HIV-1 replication.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.19453 |
| Date | January 2003 |
| Creators | Bériault, Véronique |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002010463, Theses scanned by McGill Library. |
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