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Biochemical characterization of rat colonic mucin species in response to Entamoeba histolytica

Colonic mucins bind to the E. histolytica Gal/GalNAc adherence lectin and inhibit amoebic adherence to and lysis of epithelial cells. High $M sb{ rm r}$ rat colonic mucins isolated from Sepharose 4B and subfractionated by Cellex-E (ECTEOLA) ion-exchange chromatography demonstrated a minor neutral and a major acidic species with distinct amino acid composition and immunogenicity. Virulent E. histolytica trophozoites elicited a generalized secretory response of both neutral and acidic mucin species which was confirmed by thin-section histochemistry. Such activity may function to deplete the host's protective layer to facilitate invasion. In rats immunized against E. histolytica, enhanced mucin secretion was demonstrated by an increase in the secretion of $ sp3$H-glucosamine labelled glycoproteins (25%) and high $M sb{ rm r}$ Sepharose 4B mucins (27%). Ion-exchange chromatography and histochemistry confirmed a pronounced and generalized secretion of both mucin species. Increased mucin secretion in immunized animals may function as a host defense mechanism to prevent invasion or aid in parasite expulsion.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.61048
Date January 1992
CreatorsTse, Sil-King
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Institute of Parasitology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001287142, proquestno: AAIMM74616, Theses scanned by UMI/ProQuest.

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