Colonic mucins bind to the E. histolytica Gal/GalNAc adherence lectin and inhibit amoebic adherence to and lysis of epithelial cells. High $M sb{ rm r}$ rat colonic mucins isolated from Sepharose 4B and subfractionated by Cellex-E (ECTEOLA) ion-exchange chromatography demonstrated a minor neutral and a major acidic species with distinct amino acid composition and immunogenicity. Virulent E. histolytica trophozoites elicited a generalized secretory response of both neutral and acidic mucin species which was confirmed by thin-section histochemistry. Such activity may function to deplete the host's protective layer to facilitate invasion. In rats immunized against E. histolytica, enhanced mucin secretion was demonstrated by an increase in the secretion of $ sp3$H-glucosamine labelled glycoproteins (25%) and high $M sb{ rm r}$ Sepharose 4B mucins (27%). Ion-exchange chromatography and histochemistry confirmed a pronounced and generalized secretion of both mucin species. Increased mucin secretion in immunized animals may function as a host defense mechanism to prevent invasion or aid in parasite expulsion.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.61048 |
Date | January 1992 |
Creators | Tse, Sil-King |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Master of Science (Institute of Parasitology.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: 001287142, proquestno: AAIMM74616, Theses scanned by UMI/ProQuest. |
Page generated in 0.0015 seconds