This investigation was carried out in order to determine the specificity of a panel of monoclonal antibodies previously generated against murine pregnancy-associated splenic natural suppressor cells and to characterize the cell surface target molecule(s) on cells that are recognized by these antibodies. The monoclonal antibody from clone 2C1.1 was purified on anti-rat Ig affinity column and used to partially characterize the antigenic determinant recognized by it. A 50 KDa target molecule was detected on pregnancy spleen cells enriched for Natural Suppressor activity but not on similar cells from the virgin animals. Experiments employing a cellular ELISA technique demonstrated that the antibody bound specifically to NS cells. It is proposed that the naturally occurring pregnancy-associated suppressor cells as defined by our specific anti-NS monoclonal antibodies may have physiological relevance in maintaining a state of immunological equilibration between the maternal and fetal environments by non-specifically downregulating potentially deleterious maternal anti-fetal immune reactions.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59562 |
| Date | January 1990 |
| Creators | Abedian, Azadeh |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Microbiology and Immunology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001232603, proquestno: AAIMM63711, Theses scanned by UMI/ProQuest. |
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