We have investigated the effects of graft-versus-host disease on T cell differentiation in the murine thymus. Flow cytometry analysis of L3T4 and Lyt-2 antigen expression on thymocytes along with immunofluorescence microscopy were employed to assess T cell phenotypes in thymuses of GVH mice. GVH reactions were induced by injecting 40 $ times$ 10$ sp6$ C57BL/6 (B6) or A strain lymphoid cells into C57BL/6 $ times$ A F$ sb1$, (B6AF$ sb1$) mice. Thymocyte populations were quantitated on different days after GVH induction. Our results demonstrate that single positive L3T4 cells are present in the murine GVH thymus, yet they have not acquired cortisone resistance, a trait normally attributed to this mature thymic subset. It appears that the GVH dysplastic thymus can support the differentiation of L3T4$ sp{+}$Lyt-2$ sp{-}$ cells however, such a thymus is unable to confer cortisone resistance upon this population.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68191 |
| Date | January 1994 |
| Creators | Kornbluth, Murray |
| Contributors | Lapp, W. S. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001397332, proquestno: AAIMM94449, Theses scanned by UMI/ProQuest. |
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