A chamber technique was developed allowing recovery of cytokines and T-cells from human DTH reactions. These cells were cloned, and were enriched, relative to the blood, for T-cells reactive to the antigen eliciting the DTH. Antigen-specific CD4+ clones, had two phenotypes; an IL-2/IFN-g producing group not providing help for Ig synthesis, and a group providing help but not producing IL-2 or IFN-g. Cytotoxicity and TNF production were found in both groups. TNF and IFN-g were present in the chambers over DTH reactions. Anergic patients demonstrated deficient mononuclear cell delivery to DTH sites which was restored to normal by co-injection of MLC supernatants with antigen. T-cell clones from such sites were similar to those from normal DTH sites. Additional experiments established that MLC supernatants contained 2 distinct factors, one restoring DTH in anergic patients, and another, which protected anergic rats from lethal peritonitis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74370 |
| Date | January 1989 |
| Creators | MacPhee, Martin James |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001167185, proquestno: AAINN66347, Theses scanned by UMI/ProQuest. |
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