The potential relationship between the Epstein-Barr Virus (EBV) and the Human Immunodeficiency Virus type 1 (HIV-1) in lines of EBV-carrying B cells that had been super-infected by HIV-1 was investigated. We found that such cells can produce high levels of viral particles, over long periods, with a minimal cytopathic effect and were less susceptible to HIV-1 infection than primary cultures of human peripheral blood mononuclear cells (PBMC). / We also investigated whether an alternative receptor, other than the well-known CD4 receptor, could permit HIV-1 infection of B cells immortalized by EBV. We detected a mechanism of complement-mediated antibody-dependent enhancement of HIV-1 infection in these cells implicating the CD4 receptor, the complement receptor type 2, and the alternate pathway of complement. / The fact that AZT is actually the most widely used drug in treating HIV-1 infections prompted us to investigate the ability of AZT to prevent infection and replication of HIV-1 in EBV-positive B cells. Three concentrations of AZT (1, 5 and 10 $ mu$M) prevented infection by one clinical isolate (334). In contrast, no such inhibitory effect was observed with each of a second clinical isolate (336) or with the HIV-III$ sb{ rm B}$ laboratory strain of HIV-1. Nevertheless, in these two particular cases, AZT significantly delayed and attenuated viral expression. / Finally, we determined that exposure of PBMC from HIV-1-infected individuals to preparations of HSV-1, HSV-2, or CMV stimulated the cells to become active producers of HIV-1 and point to a possible role for these viruses as co-factors in the pathogenesis of AIDS.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74257 |
| Date | January 1989 |
| Creators | Tremblay, Michel, 1955- |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001068853, proquestno: AAINN63407, Theses scanned by UMI/ProQuest. |
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