The transmembrane potential changes ((DELTA)(PSI)) induced by the sensitizing cancer extract in leukocytes from patients with cancer were measured as part of their transmembrane signalling. Cancer extracts induce (DELTA)(PSI) changes in sensitized leukocytes, initiating the cascade of reactions that lead to leukocyte nonadherence to glass. Monocytes armed with cytophilic antitumor antibodies recognized the organ-specific cancer neoantigen (OSN) in extracts of allogeneic cancer and released leukotriene mediators that affected themselves and bystander leukocytes. Whereas the monocyte response is not MHC restricted, the T-cell response in the LAI and (DELTA)(PSI) assay is limited to extracts of autologous cancer and restricted by class I MHC antigens. (DELTA)(PSI) changes are seldom observed in leukocytes from patients with advanced cancer to either OSN or leukotrienes. The evidence suggests that these leukocytes are activated in vivo and are therefore refractory to a second in vitro stimulus. Increasing intracellular cyclic AMP restores transmembrane signalling and LAI.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.71863 |
| Date | January 1983 |
| Creators | Shenouda, George. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000218386, proquestno: AAINK66582, Theses scanned by UMI/ProQuest. |
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