Gut associated lymphoid cells are modulated by gut hormones. We postulated that lymphokine-associated-killer (LAK) cell cytotoxicity of lymphocytes from the gut mucosa may be increased by substance P (SP). / Intestinal lamina propria mononuclear cells (LPMC) and colonic cancer cells were isolated from operative specimens by mechanical and enzymatic dissociation methods. Effector LAK cells were induced by culturing LPMC with 250 u/ml of recombinant interleukin-2 (IL-2). Substance P (10$ sp{-5}$M) was added to the culture. Targets consisted of fresh colon cancer cells, HT-29 and control cell lines. After 4 days of incubation, cytotoxicity was measured using a 4-hour $ sp{51}$Cr release assay. / LAK cells showed moderate cytotoxicity against HT-29 and none against fresh colon cancer cells. LAK cells + substance P showed moderate cytotoxicity against HT-29 and strong cytotoxicity against fresh cancer cells. Cytotoxicity $ pm$ SEM at various effector-to-target (E:T) ratios was: (* denotes p $<$ 0.05 compared with above).(UNFORMATTED TABLE OR EQUATION FOLLOWS) vbox{ halign{# hfil&& enspace# hfil cr &Effector&Target&N&5:1&10:1&25:1&50:1 cr cr&LAK&HT-29&7&2.8$ pm $0.5&11.6$ pm$1.0&12.3$ pm$2.3&17.8$ pm$4.2 cr&LAK+SP&HT-29&11&1.2$ pm $0.5& enspace 4.1$ pm $0.9& enspace 9.3$ pm$1.0&16.9$ pm$3.1 cr&LAK+SP&Fresh Ca&8&5.6$ pm$1.2&12.6$ pm$3.3&38.3$ pm$6.3*&59.4$ pm$9.5* cr&LAK&Fresh Ca&11&0.0$ pm$0.0*& enspace 0.1$ pm$0.1*& enspace 0.1$ pm$0.1*& enspace 0.5$ pm$0.5* cr}} TABLE/EQUATION ENDS)Substance P significantly increases LAK cell cytotoxicity against fresh colon cancer cells, but not against cultured cells.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56993 |
| Date | January 1992 |
| Creators | Flageole, Hélène |
| Contributors | Lirudel, Judith (advisor), Brown, Rae A. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001319271, proquestno: AAIMM87737, Theses scanned by UMI/ProQuest. |
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