$ sp1$H NMR (1D and 2D) studies on human cerebrospinal fluid (CSF) are used to characterize normal CSF. Methyl malonic acid (MMA) is found (ca 154 $ mu$M) only in CSF from a vitamin B$ sb{12}$ deficient patient. / $ sp{31}$P NMR studies on the effects of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) on perfused SKI-1 glioma cells show a transient intracellular alkalosis of a similar magnitude, (0.13 $ pm$ 0.05) pH units, to that observed in vivo. This pH alteration may be related to Na$ sp+$ influx. / Multiple-quantum (MQ) filtered $ sp{23}$Na NMR experiments are described using the multipole formalism. A triple quantum filter (TQF;$ vert$3/2$>$ $ leftrightarrow$ $ vert-$3/2$>)$ is more selective for intracellular sodium (Na$ sp+ sb{ rm in})$ than the DQF$( vert$3/2$>$ $ leftrightarrow$ $ vert-$1/2$>$ and $ vert$1/2$>$ $ leftrightarrow$ $ vert-$3/2$>),$ under identical conditions, in human red blood cells. Studies on model solutions of bovine serum albumin (BSA) suggest that at constant ionic strength, the MQ filtered signal is directly proportional to the single quantum signal. Dysprosium tripolyphosphate, Dy(PPP$ sb{ rm i}) sb2 sp{7-},$ is used to discriminate between intracellular and extracellular Na$ sp+$ in neuroblastoma and human red blood cells.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.39323 |
| Date | January 1992 |
| Creators | Commodari, Fernando |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001284272, proquestno: NN74813, Theses scanned by UMI/ProQuest. |
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