The purpose of this investigation was to determine whether commercial processing of cow's milk leads to cholesterol oxidation. Pasteurized whole milk, ultrahigh temperature milk, canned evaporated milk, skim milk and instant nonfat dry milk were purchased from a local supermarket. Fresh raw milk was obtained from an area farm. Thin layer chromatography (TLC) was used for the initial analysis of the unsaponified total lipid extracts of the samples. Sterols were isolated and eluted from chromatoplates for gas chromatography-mass spectrometry (GC-MS) analysis. TLC and GC data indicate the cholesterol in liquid milk is stable during commercial pasteurization, sterilization and evaporation and to further heating of the milk for 12 hours at 85(DEGREES)C. TLC data indicate the presence of 7-hydroxycholesterol in instant nonfat dry milk. However, GC-MS analysis did not resolve this compound. The results of this investigation suggest that the spray-drying process may catalyze cholesterol oxidation but further analytical and experimental investigation is required to confirm such a conclusion. / Source: Dissertation Abstracts International, Volume: 47-07, Section: B, page: 2842. / Thesis (Ph.D.)--The Florida State University, 1986.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_75886 |
| Contributors | CLEVELAND, MAE EVELYN ZIGLER., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 103 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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