The effects of the incorporation of trans-fatty acids in the diet of rats on the mutagenic potential of benzo(a)pyrene (BP), 2-aminofluorene (AF), 2-acetylaminofluorene (AAF) and 2-anthramine (AA) were evaluated in the Ames Assay incorporating rat hepatocytes and liver cell homogenates (S-9 fraction) as the metabolic activating systems. The hepatocytes and S-9 fractions were derived from Aroclor or BHT induced and non-induced animals fed the experimental and control diets, 45% to 55% and 0% trans-fatty acids of a 15% fat diet, respectively. / Benzo(a)pyrene, 2-acetylaminofluorene and 2-aminofluorene were mutagenic in the S-9 assays from Aroclor and BHT induced animals fed the control and trans-fatty acid diets. In the S-9 assays from non-induced animals fed the control and trans-fatty acid diets, benzo(a)pyrene and 2-acetylaminofluorene were weakly mutagenic. Aroclor was a more effective inducer of benzo(a)pyrene and 2-acetylaminofluorene mutagenicity than BHT. The effects of the inducers, Aroclor and BHT, on the mutagenic potential of 2-aminofluorene was negligible. / The effects of diet on mutagenesis were few. The mutagenic potential of benzo(a)pyrene was significantly decreased in the S-9 assays from Aroclor induced animals fed the trans-fatty acid diet when compared to S-9 assays from Aroclor induced animals fed the control diet. The mutagenic response from 2-acetylaminofluorene was significantly higher in S-9 assays from BHT induced animals fed the trans-fatty acid diet than S-9 assays from animals fed the control diet. In assays from non-induced animals, a higher concentration of 2-acetylaminofluorene was required for mutagenesis with S-9 from trans-fatty acid fed animals than S-9 from animals fed the control diet. / In the hepatocyte assays, benzo(a)pyrene, 2-anthramine and 2-aminofluorene did not produce a mutagenic response. Effects from the inducers Aroclor and BHT or diet on the mutagenicity of benzo(a)pyrene, 2-anthramine and 2-aminofluorene were not observed in the hepatocyte assay. The S-9 fractions were more effective than hepatocytes in the metabolic activation from benzo(a)pyrene and 2-aminofluorene. / Source: Dissertation Abstracts International, Volume: 44-10, Section: B, page: 3042. / Thesis (Ph.D.)--The Florida State University, 1983.
| Identifer | oai:union.ndltd.org:fsu.edu/oai:fsu.digital.flvc.org:fsu_75194 |
| Contributors | PONDER, DEBRA LYNN., Florida State University |
| Source Sets | Florida State University |
| Detected Language | English |
| Type | Text |
| Format | 127 p. |
| Rights | On campus use only. |
| Relation | Dissertation Abstracts International |
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