Arterial Glycosaminoglycans (GAGs) have gained importance in artherogenesis due to their ability to trap lipid inside the vessel wall. Atherosclerotic lesions have displayed an altered GAG content and distribution. Diabetes is a recognized risk factor for atherosclerosis, but no information is available on the arterial GAGs in human diabetes. To improve our understanding of the atherogenic proccss we examined GAGs in normal and atherosclerotic intima of nondiabetic and type-II diabetic humans. Intima was stripped from the autopsy samples of thoracic aortas, normal and plaque areas were separated. GAGs were isolated by delipidatlon, proteolytic digestion, and precipitation. They were assayed biochemically and their distribution evaluated by electrophoresis and densitometry. Results indicate a significant decrease in total GAGs and a change in GAG distribution in plaques of nondiabetics. Similar changes of lesser magnitude were found in normal intima of diabetics, while changes in plaque areas were more pronounced. This indicates that changes in arterial GAGs precede the development of lesions in diabetes and may be important in atherogenesis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.61187 |
| Date | January 1992 |
| Creators | Wasty, S. Fasahat |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001276976, proquestno: AAIMM74865, Theses scanned by UMI/ProQuest. |
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