Allergen-induced rhinitis is associated with the recruitment and activation of inflammatory cells, particularly eosinophils and CD4 + T cells into the nasal mucosa. Monocyte chemotactic proteins (MCPs) have been shown to induce chemotactic activity in these particular cell types under in vitro assay conditions. To assess the contribution of MCPs in the recruitment of inflammatory cells in vivo, we investigated the allergen-induced late response in subjects with allergic rhinitis. Using immunocytochemistry and in situ hybridization, we demonstrated a constitutive expression of MCP-1, -3 and -4, of which MCP-3 and -4 were significantly increased in the nasal mucosa following allergen provocation. This upregulation of MCP-3 and 4 immunoreactivity in response to allergen, was reduced in patients pretreated with topical corticosteroids. Colocalization experiments revealed that the majority of MCP-positive cells were macrophages. The results of this study suggest that allergen-induced rhinitis is associated with an increased expression of MCP-3 and -4, which may be closely related to the influx of inflammatory cells and may thus contribute to the pathogenesis of allergic rhinitis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21526 |
| Date | January 1998 |
| Creators | Christodoulopoulos, Pota. |
| Contributors | Hamid, Outayba (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001650450, proquestno: MQ50737, Theses scanned by UMI/ProQuest. |
Page generated in 0.0018 seconds